SrasV1, Main, Exploration, bibRecord, 003082

Isolation of inhibitory RNA aptamers against severe acute respiratory syndrome (SARS) coronavirus NTPase/Helicase.

Identifieur interne : 003082 ( Main/Exploration ); précédent : 003081; suivant : 003083

Isolation of inhibitory RNA aptamers against severe acute respiratory syndrome (SARS) coronavirus NTPase/Helicase.

Auteurs : Kyoung Jin Jang [Corée du Sud] ; Na-Ra Lee ; Woon-Seok Yeo ; Yong-Joo Jeong ; Dong-Eun Kim

Source :

Biochemical and biophysical research communications [ 1090-2104 ] ; 2008.

RBID : pubmed:18082623

Descripteurs français

KwdFr :
- Aptamères nucléotidiques (administration et posologie), Aptamères nucléotidiques (génétique), Extinction de l'expression des gènes, Nucleoside-triphosphatase (antagonistes et inhibiteurs), Nucleoside-triphosphatase (génétique), RNA helicases (génétique), Virus du SRAS (génétique), Virus du SRAS (physiologie).
MESH :
- administration et posologie : Aptamères nucléotidiques.
- antagonistes et inhibiteurs : Nucleoside-triphosphatase.
- génétique : Aptamères nucléotidiques, Nucleoside-triphosphatase, RNA helicases, Virus du SRAS.
- physiologie : Virus du SRAS.
- Extinction de l'expression des gènes.

English descriptors

KwdEn :
- Aptamers, Nucleotide (administration & dosage), Aptamers, Nucleotide (genetics), Gene Silencing, Nucleoside-Triphosphatase (antagonists & inhibitors), Nucleoside-Triphosphatase (genetics), RNA Helicases (genetics), SARS Virus (genetics), SARS Virus (physiology).
MESH :
- chemical , administration & dosage : Aptamers, Nucleotide.
- chemical , antagonists & inhibitors : Nucleoside-Triphosphatase.
- chemical , genetics : Aptamers, Nucleotide, Nucleoside-Triphosphatase, RNA Helicases.
- genetics : SARS Virus.
- physiology : SARS Virus.
- Gene Silencing.

Abstract

Recent outbreak of Severe Acute Respiratory Syndrome (SARS) that caused almost 800 victims requires a development of efficient inhibitor against SARS coronavirus (SCV). In this study, RNA aptamers against SCV NTPase/Helicase (nsP10) were isolated from RNA library containing random sequences of 40 nts using in vitro selection technique. Nucleotide sequences of enriched RNA aptamer pool (ES15 RNA) contain AG-rich conserved sequence of 10-11 nucleotides [AAAGGR(G)GAAG; R, purine base] and/or additional sequence of 5 nucleotides [GAAAG], which mainly reside at the loop region in all the predicted secondary structures. Isolated RNAs were observed to efficiently inhibit double-stranded DNA unwinding activity of the helicase by up to approximately 85% with an IC(50) value of 1.2nM but show a slight effect on ATPase activity of the protein in the presence of cofactor, poly (rU). These results suggest that the pool of selected aptamers might be potentially useful as anti-SCV agents.

DOI: 10.1016/j.bbrc.2007.12.020
PubMed: 18082623

Affiliations:

Corée du Sud

Le document en format XML

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<term>Extinction de l'expression des gènes</term>
<term>Nucleoside-triphosphatase (antagonistes et inhibiteurs)</term>
<term>Nucleoside-triphosphatase (génétique)</term>
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<front><div type="abstract" xml:lang="en">Recent outbreak of Severe Acute Respiratory Syndrome (SARS) that caused almost 800 victims requires a development of efficient inhibitor against SARS coronavirus (SCV). In this study, RNA aptamers against SCV NTPase/Helicase (nsP10) were isolated from RNA library containing random sequences of 40 nts using in vitro selection technique. Nucleotide sequences of enriched RNA aptamer pool (ES15 RNA) contain AG-rich conserved sequence of 10-11 nucleotides [AAAGGR(G)GAAG; R, purine base] and/or additional sequence of 5 nucleotides [GAAAG], which mainly reside at the loop region in all the predicted secondary structures. Isolated RNAs were observed to efficiently inhibit double-stranded DNA unwinding activity of the helicase by up to approximately 85% with an IC(50) value of 1.2nM but show a slight effect on ATPase activity of the protein in the presence of cofactor, poly (rU). These results suggest that the pool of selected aptamers might be potentially useful as anti-SCV agents.</div>
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Serveur d'exploration SRAS

Isolation of inhibitory RNA aptamers against severe acute respiratory syndrome (SARS) coronavirus NTPase/Helicase.

Isolation of inhibitory RNA aptamers against severe acute respiratory syndrome (SARS) coronavirus NTPase/Helicase.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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